The Bioscan, 1(1-4) 013-017,2006
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
PARTIAL PURIFICATION OF THE ENZYME INVOLVED IN BIOCONVERSION OF
ARTEANNUIN B TO ARTEMISNIN FROM A STREPTOMYCES SP.
PARCH SREENIVASA RAO, LAKSHMI SREENIVAS, Y. PRAMEE DEVI & M. LAKSHMI NARASU*
Centre of Biotechnology, Institute of Science and Technology
Jawaharlal Nehru Technology University, Hyderabad, INDIA
Email :mangmoori@rediffmail.com
KEY WORDS

Artemisinin
Artennin-B
Artemisia annua
Streptomyces sp
Bioconversion

Received on: 13.09.2006

Accepted on: 21.11.2006



*Corresponding
 Author
ABSTRACT

Artemisinin and its derivatives are the most rapidly acting antimalarial drugs effective against falciparum malaria including multidrug resistant infection. An enzyme catalyzing the bioconversion of arteannin-B, a biogenetic precursor of artemisinin to the later is partiallt purified from a soil isolate, Streptomyces sp. Crude cell free extract of a 72 h old culture of Streptomyces sp. on incubation with the precursor arteannuin B had shown bioconversion of 17.64% to artemisinin on molor basis with a specific activity of 0.11 units/mg. Partial pruification of the enzyme by ammonium sulfate precipitation and ion exchange chromatography has resulted in .5.60 fold increase of specific activity with 64.71% of bioconversion