The Bioscan, 1(1-4) 019-025,2006
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
MICROBIAL TRANSFORMATION OF GALLOTANNINS TO GALLIC ACID, AN 
INTERMEDIATE PRODUCT OF TRIMETHOPRIM, A BROAD SPECTRUM ANTIBOITIC
N. LOKESWARI, K. JAYA RAJU, M. NARSIMHA RAO* AND C. AYYANNA
Centre of Biotechnology, Department of Chemical Engineering.
Andhra University, Visakhapatnam- 530 003, INDIA
Email :mnrmd_au@yahoo.com
KEY WORDS

Gallotannins
Trimethoprim
Gallic Acid
Tannase

Received on: 20.07.2006

Accepted on: 07.12.2006





*Corresponding
 Author


ABSTRACT

A method for producing Gallic acid by microbiological hydrolysis of the tannins of tripods powder is described in the present work. Hydrolysis of gallotanins of the substrate to Gallic acid by aspergilus niger MTCC 282 was studied. A simple extraction procedure is used. Fungal mycelia pre-induced with 5 g/l gallotanin was used as inocolums. Optimal values for various physico-chemical parameters including substrate concentration, inocolum levels, pH, temperature, fermentation, inocolum age, agitatioin, gallotanin concentration nultritional source and metal ion were determined. The yield of Gallic acide with respect to gallotannins present in the substrate is estimated. Yield of Gallic acid are about 82% with respect to gallotannin concentration, which suggests that this method ix exploitable industrially for manufacturing Trimethoprim drug.

Currnet total requirment of Gallic acid is around 8,000 tones per year all over the world. Conventionally Gallic acid is being produced by acid hydrolysis of tannin rich source. But this technology has serval disadvantages regarding cost, yield and purity of the product. This technology alos causes a lot of environmental pollution being a chemical process. The present technique of conversion of teri tannins to Gallic acid using fungal mycelia viz, aspergillus niger MTCC 282, being purely a bioconversion is free from pollution with more purity of product.