The Bioscan, 2 (1) 001-07,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
3D STRCUTURAL MODELLING FOR PROCATECHUATE 3,4 DIOXYGENASE
FROM PSEUDOMONAS CEPACIA BASED ON TEMPLATE OF M CHAIM 
FROM P.AERUGINOSA
D.JAYASREE*, J. VENKATESHWARA RAO*,D. VENKATA KRISHNA RAO*, K.K. KUMAR*
P.B.K. KISHORE***, H.A. NAGARAJARAM**, Y. PRAMEELA DEVI*, M. LAKSHMI NARASU*
* Centre of Biotechnology, Institute of Sceince & Technology, JNTU, Kukatpally- 500 085, Hyderabad.
** Centre of DNA and Finger Printing Diagnostics, Nacharam - Hyderabad.
***Department of Genetics, Osmania University, Hyderabad- 500 007

KEY WORDS

Multiple sequence
alignment
Ramachandran Plot
Joy alignment
Steriochemical
equality


Received on: 21.10.2006

Accepted on: 21.07.2007





*Corresponding
 Author

ABSTRACT

The 3D Model of Protocatethuate 3,4 dioxygenase beta chain from Psedomonas cepacia was built, based on X-ray crystallographic structure of Protocatethuate 3,4 dioxygenase M chain from Pseudomonas aeruoginosa. Dioxygenase catalyses the cleavage of molecular oxygen with subsequent incorporation of both oxygen atoms into organic substrates. Some of the dioxygenase from bacteria catalyse critical ring-opening step in biodegradation of aromatic compounds. These bacterial enzymes contain non-hme ferric iron as the sole cofactor. Protocatethuate 3,4 dioxygenase (3,4PCD) was one of such enzyme recognized which catalyses the intradiol cleavage of protocatechic acid by oxygen to produce a-carboxy-cis-cis-muconic acid. Studies on 3,4 (PCD) found in Pseudomonas aeruginosa is an oligomer with relative molecular mass (587K). The homology modelling is done based on the programmes to annotate protein sequence aligment with 3D structural features which helps to understand the conversation of amino acid in the specfic local environment.