The Bioscan, 2 (1) 015-020,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
GENOTOXIC EFFECTS OF CARBARYL ON INDIAN CRICKET FROG
LIMNONECTES LIMNOCHARIS OF WESTERN GHATS
G.R. KRISHNAPPA* AND VENKATESHWARLU
Department of Aplplied Zoology, School of Bio Sciences,
Jnana Sahyadri, Kuvempu University, Shankaraghatta - 577 45, INDIA.
E-mail: krishnappa102@yahoo.com

KEY WORDS

Genotoxicity
L. limnocharis
Chromosomal
aberrations
Micronnucleans assay
Sperm morphology
assay


Received on: 19.10.2006

Accepted on: 31.01.2007


*Corresponding
 Author

ABSTRACT

Tracing many reasons, pesticidal cause is gaining momentum for the decline of amphibian population. As there are no report on the reffects of pesticides on amphibian fauna of Wester Ghats, carbaryl a carbamate pesticide was used to test their genotoxic effect on L. limnocharis. The pilot toxicity studies have revealed that LD50 was estimated to be 125mg/kg b. wt. at 96 h by an intraperitnoeal injection. The median lethal and sub lethal doses 125, 93.75, 62.50 and 32.25 mg/kg body weight equivalent to LD50 and 3/4th, 1/2nd, 1/4th of the LD50 were employed to treat the animals for further studies. Protocols such as chromosomal aberration micronucleus test and sperm morphology assay were used to evaluate the genotoxicity. The frequency of chromosomal abreration are not significant even at higher dose, micronucleus and sperm abnormalities were significant more in higher doses of pesticide exposure during 72 and 96 h treated group compared to control.