The Bioscan, 2 (1) 021-024,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
QUALITATIVE DETECTION OF LEAD IN FORTIFIED WATER SAMPLE
BY A PAPER CHROMATOGRAPHIC ENZYME INHIBITION TECHNIQUE
G. SEETHAMMA* & N. V. NANDA KUMAR
Division of Environmnental Biology, Department of Zoology,
S.V. University, Tirupati- 517 502, A.P., INDIA.
E-mail: dr.seetha_zoology@yahoo.com

KEY WORDS

Water analysis
Chick brain
Enzyme
Lead



Received on: 19.10.2006

Accepted on: 31.01.2007


*Corresponding
 Author

ABSTRACT

Chick heads thrown out as biowaste were employed as a enzyme source for the detection of lead compound by an enzyme inhibtion technique on paper chromatograms. The lead compounds (lead nitrate and lead acetate) appeared as white spots on a pink back ground due to the inhibition of chick brain succinate degydrogenase employing a chromogenic regant mixture containing sodium succinate substrate, (2-(4-lodophenyle)-3-(4-nitrophenyl)-5 phenyl tetrazolium chloride and N-methyl phenazonium metho sulphate. The minimum detectable amount were about 5úg of lead nitrate and 5úg of lead acetate. Many other animal succinate dehydrogenases also gave inhibition spot under the same condition on chromatograthic paper but they were less sensitive than chick brain succinate dehydrogenase. Wate sample was fortified with lead acetate and were detected by enzyme inhibition technique.