The Bioscan, 2 (1) 025-029,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
ISOLATION AND CHARACTERIZATION OF A MODERATERLY HALOPHILIC
PROTEASE PRODUCING PSEUDOMONAS SP. FROM BRINE SAMPLES OF
A COASTAL AREA SOLAR SALTERN
P. LAKSHMI SREENIVAS, PARCHA SREENIVASA RAO, Y. PRAMEELA DEVI AND M. LAKSHMI NARASU*
Centre of Biotechnology, Institute of Science and Technology, Jawarlal Nehru Technology University, Hyderabad - 500 085. India E-mail: mangamoori@rediffmail.com

KEY WORDS

Moderately
Halophillic
Protease
Extracellular
Saltern
Pseudomonas



Received on: 19.10.2006

Accepted on: 31.01.2007



*Corresponding
 Author

ABSTRACT

Screening of the brine samples of solar has yielded one moderately halophillic protease producing bacteria. The bacterial isolate has been indentified as a Pseudomonas sp. and it produced extracellular protease and an amylase. Maximal protease production was detected at the early stationary phase. The enzyme showed the highest activity at 40°C and pH 8.0 in 15% Nacl. The enzyme was active over a range of salt (0-25%), pH (6.5-10.0) and temperature (20°C-55°C). These broad ranges of tolerance of the enzyme indicate potential application of this enzyme in fish sauce fermentation and detergent formulations.