The Bioscan, 2 (1) 063-066,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
IN- VITRO REGENERATION AND CALLUS FORMATION FROM DIFFERENT PART
OF SEEDLING OF MUCUNA PRURIENS BAK A VALUABLE MEDICINAL PLANT
SHALINI PATEL*, ANITA MEHTA*, AND R.K. PANDEY**
*Department of Biotechnology, Ranchi Women's College, Ranchi - 834 001 **Department of Botony, Ranchi University, Ranchi - 834 008. E-mail: shalini_rch@rediffmail.com

KEY WORDS

Muccuna pruriens
L-DOPA
Green callus
White callus
Regeneration



Received on: 13.11.2006

Accepted on: 06.01.2007



*Corresponding
 Author

ABSTRACT

Callus proliferation was studies on cotyledon, leaf and stem explant of Muccuna pruriens Bak cultured on Murashige and Skoog's medium (MS) supplemented with 2,4-D, IBA, NAA and BAP alone or in combination. Light brown callus formation was followed by formation of milky white callus on the surface of young excised shoots and leaf tissues of Mucuna pruriens. Sometimes green callus was also observed. Development of root and stem with leaves were investigated from excised stem, leaf and cotyledon tissues.