The Bioscan, 2 (2) 083-087,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
CHICK BRAIN ENZYME INHIBTON METHOD FOR DETECTION OF LEAD
COMPOUNDS BY THIN LAYER CHROMATOGRAPHY.
G.SEETHAMMA* AND N.V. NAND KUMAR
Divition of Environmental Biology, Department of Zoology, S.V. University, Tirupati - 517 502, A.P., INDIA. E-mail: dr.seetha_zoology@yahoo.co.in

KEY WORDS

Chick brain
Lead
Succinate
dehydrogenase
Thin layer
chromatography



Received on: 02.12.2006

Accepted on: 06.03.2007



*Corresponding
 Author

ABSTRACT

Chick brain succinate dehydrogenase was employed as a biosource for the detaction of lead compound from industrial water sample by an enzyme inhibition technique on thin layer chromatograms. The lead compounds (lead nitrate abd lead acetate) appeared as white spots on a pinkb back ground due to the inhibition of chick brain succinate dehydrogenase employing a chromogenic reagent mixture containing sodium succinate substrate, (2-(4-lodophenyl) -3 (4- nitrophenyl) -5 phenyl tetrazolium chloride and N-methyl phenasonium metho sulphate. Many other animal succinate dehydrogenases also gave inhibition spot under the same condition on micro thin layer chromatographic plates but they were less sensitive than chick brain succinate dehydrogenases. Industrial water sample was collected from scrab smelter plant and was used for detection of lead compound by enzyme inhibition technique.