The Bioscan, 2 (2) 121-124, 2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
RESPONSE OF EMBRYOS OF SOME VARIETIES OF RICE FOR THEIR CALLUSING
AND DIFFERENTIATION
R.P.SINGH*, H.P.SHARMA 1 AND S.K.SINHA 2
Department of Botany, R.S. More College, Govindpur-828 109, Dhanbad. 1 University Department of Botany, Ranchi University, Ranchi - 834 008. 2 Department of Zoology, R.S. More College, Govindpur- 828 109, Dhanbad.

KEY WORDS

Callusing
Differentiation
in-vitro
Oryza sativa L



Received on: 17.11.2006

Accepted on: 21.01.2007


*Corresponding
 Author

ABSTRACT

Rice is the main food of most of the countries of the world. To cope with the population explosion, conventional method of rice production seems inadequate. Hense, for this purpose; tissue culture technology has been introduced. Before applying any advanced study, such as anther culture, protoplast culture or genetic engineering etc., it is essential to work on the rice varieties from its embryo level. In this perspective, four varieties of rice had been undertaken for the response of their embryos on different concentrations of 2,4D, for their callusing and upon different concentrations of IAA and KN for differentiation. Out of the four varieties of rice, Tulsi and Kanak embryos showed the best response for their callusing at the strength 6 mg/l of 2,4D - 40% and 22% respectively, which is followed by Pusa-Basmati and Birsa 101 embryos. Almost all the varieties showed their callusing more or less at all the strength of 2,4D taken into investigation. But the strength 6 mg/l was the best strength of 2,4D as upon this strength, all the varieties showed their callusing response. On the other hand, embryo derived callus when transferrred to differentiating medium MS + IAA (2 mg/l) + KN(4mg/l) gave the best result.