The Bioscan, 2 (4): 269-274,2007
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
IN VITRO  REGENERATION OF PIPER LONGUM FROM AXILLARY BUDS: A 
BIOPHARMACEUTICALLY POTENT MEDICINAL PLANT
A. C. DEKA* AND M. C. KALITA
Department of Biotechnology, Gauhati University,
Guwahati - 781 014, Assam, INDIA
E-mail: mckalita@sify.com

KEY WORDS

Axillary bud 
Multiplication
Micropropagation
Piper longum
Received on: 27.03.2007 Accepted on: 29.07.2007 *Corresponding Author

ABSTRACT

Multiple shoots regeneration of Piper longum through axillary bud proliferation was established. Murashige and Skoog (MS) medium containing 1.0 mg/L N6-benzylaminopurine(BAP), 0.5 mg/L a-Naphthaleneacetic acid (NAA) , 20 mg/L AdSO4 and 100 mg/L ascorbic acid developed a mean of 15.6 numbers of shoots per node explant and was superior to Gamborg et al., (B5) and Schenk and Hilderbrandt (SH) medium. Shoots developed were rooted the best in MS medium with 0.5 mg/L NAA (produced a mean of 4 number of roots per shoot within 6 days of culture) when compared to that of B5 and SH. The plantlets exhibited 90% survival in field conditions and were similar in morphological features to that of the source plant.