The Bioscan,3(2): 139-146, 2008
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
SOME HISTOCHEMICAL OBSERVATIONS ON THE OVARIAN STROMAL 
INTERSTITIAL CELLS DURING ANOESTROUS AND OESTROUS IN INDIAN 
LEAF-NOSED BAT HIPPOSIDEROS SPEORIS (SCHNEIDER)
M. S. SASTRY* AND DHARNA TEMBHARE
Department of Zoology,
Rashtrasant Tukdoji Maharaj Nagpur University, Nagpur - 440 033
E-mail: sastry_nidhi@rediffmail.com


KEY WORDS
Stromal interstitial 
cells
Bat
Steroidogenic
enzymes
Received on: 19.08.2007 Accepted on: 27.12.2007 *Corresponding Author

ABSTRACT

Hipposideros speoris is an insectivorous monotocous and monoestrus bat breeding once in a year. The female genitalia were morphologically bilaterally symmetrical but sinistral dominance existed. Stromal type interstitial gland cells (Igc) were observed in much of the cortex and medulla in both the functional and non-functional ovaries but the present work is mostly from the functional ovary. During anestrous stromal cells were less differentiated, compact and mostly spindle-shaped but with the onset of oestrous phase these cells become swollen and sparsely distributed and there is an increase in the vascularization. The cellular localization and relative activity of lipid and various enzymes (3ß, 17ß-HSD; G-6-PD, SDH, alkaline and acid phosphatase) have been histochemically demonstrated. Thus during anoestrous the lipid appeared as abundant coarse granules while during oestrous the granules were smaller in size and sparsely distributed. The alkaline and acid phosphatase enzymes essential for a number of metabolic activity were also demonstrable in varying degree and their intensity paralleled the accumulation of lipids. Correlative to the lipid profile the steriodogenic enzymes 3ß, and 17ß-HSDH showed differential staining characteristic. The histochemical studies of succinate dehydrogenase (SDH) and glucose – 6 – phosphate dehydrogenase (G-6-PD) in conjunction with lipid and steroidogenic enzymes suggest a major role of stromal compartment of ovary in hormone synthesis probably the androgens.