The Bioscan, 3(4): 373-376, 2008
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
DETECTION, IDENTIFICATION AND SEPARATION OF DICHLORVOS (DDVP) BY 
ENZYMATIC METHOD
UMA MAHESWARA RAO GANGURU AND YALAVARTHY PRAMEELA DEVI*
Environmental Biotechnology Laboratory, Center for Biotechnology,
Institute of Science and Technology, Jawaharlal Nehru Technological University,
Hyderabad - 500 085 (A.P.) INDIA.
E-Mail: prameeladeviy@yahoo.co.in

KEY WORDS
Dichlorvos
Succinate Dehydrogenase (SDH)
Escherichia coli
Chick liver
Egg albumin Received on: 05.06.2008 Accepted on: 14.10.2008 *Corresponding Author

ABSTRACT

The present paper describes a simple, low cost, rapid, viable and sensitive enzymatic method for detection of Dichlorvos (DDVP) at microgram level. The important principle involved in this method is the biochemical reaction between the DDVP and its inhibition caused on the succinate dehydrogenase (SDH). Wattman No – 3 filter paper was used as a matrix. Escherichia coli, chicken liver and egg albumin were separately homogenized, lyophilized and these lyophilized powders were used as an enzyme source. For the detection of DDVP, 2- (4- Ido-phenyl)-3-(4-nitrophenyl)-5 phenyl tetrazolium chloride (INT) and n-methyl phenazonium methosulphate (PMS) mixture were used as a chromogenic reagent. Wattman No - 3 filter paper was used to prepare both enzyme and substrate strips using lyophilized powder and chromogenic reagent. Standard samples of DDVP were prepared and applied on enzyme strip with the help of a graduated micro capillary. Two micrograms of DDVP was detected successfully by using this method. For separation of DDVP, micro thin layer chromatographic methods were used by using an organic solvent mixture with the same enzyme inhibition principle.