The Bioscan, 4(3): 387-393, 2009
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
BIODEGRADATION OF NAPHTHALENE BY IMMOBILIZED PSEUDOMONAS 
FLUORESCENS KCP1
N. CHANDRASEKHAR11 AND C. S. KARIGAR*
1Department of Chemistry, Shridevi Institute of Engineering and Technology, Tumkur - 572 106
Bioremediation Laboratory, Biochemistry Division, Department of Biochemistry,
Central College Campus, Bangalore University, Bangalore - 560 001, INDIA
E-mail: karigar@bub.ernet.in


KEY WORDS
Naphthalene 
Pseudomonas fluorescens 
KCP1 
Surfactants
Salicylic acid Immobilization
Received on: 21.05.2009 Accepted on: 27.08.2009 *Corresponding Author

ABSTRACT

Pseudomonas fluorescens KCP1 species metabolized naphthalene as the sole source of carbon and energy. Salicylic acid was identified as one of the metabolic intermediate during the biodegradation of naphthalene based on TLC and enzyme analysis. Pseudomonas fluorescens KCP1 cell free extracts exhibited salicylate hydroxylase enzyme with a specific activity of 0.15µmoles min-1mg-1 of protein. The degradation rates were determined in the presence and in the absence of commercial surfactants. The Pseudomonas fluorescens KCP1 species was immobilized in alginate and agar matrices. Both the matrices were stable with degradation ability for a period of 45 days and 31 days respectively.