The Bioscan, 4(3): 399-403, 2009
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
BIODEGRADATION OF FERULIC ACID BY BACILLUS PUMILUS ZYJ1-2
JAGANNATH C. KADAKOL1 AND CHANDRAPPA M. KAMANAVALLI*   
Department of Chemistry, Karnatak Science College, Dharwad - 580 001, INDIA
E-mail: ckamanavalli@yahoo.co.in


KEY WORDS
Biodegradation 
Ferulic acid
Bacillus pumilus strain zyj1-2
Received on: 29.06.2009 Accepted on: 11.08.2009 *Corresponding Author

ABSTRACT

A soil bacterium Bacillus pumilus strain zyj1-2 was isolated and grown in liquid media containing ferulic acid as a sole source of carbon and energy. Thin layer chromatography and uv-spectrophotometry showed that 4-vinylguaiacol, by decarboxylation, which was further metabolized to vanillin, then oxidized to vanillic acid and protocatechuate, which undergoes ortho-ring cleavage. The cell-free extract of Bacillus sp grown on ferulic acid contained the activity of ring cleavage dioxygenase protocatechuate 3,4-dioxygenase was detected. The organism also utilized 4-vinylphenol, p-coumaric acid, p-hydroxybenzaldehyde, and p-hydroxy benzoic acid as growth substrates.