The Bioscan, 4(3): 405-408, 2009
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
RELATIONSHIP BETWEEN PLASMA GLUCOSE, ISLET CELLS, LIVER 
GLYCOGEN AND ABDOMINAL FAT IN HIBERNATING TURTLE 
LISSEMYS PUNCTATA
VIDYA R. CHANDAVAR, PRAKASH R. NAIK* AND H. VIMALA 
Endocrine Laboratory, Department of Studies in Zoology, University of Mysore,
Manasagangorti, Mysore - 570 006, INDIA
E-mail: prakashrnaik@yahoo.co.in


KEY WORDS
Glucagon-IR
Hibernation
Insulin-IR
Liver glycogen
Plasma glucose
Received on: 21.05.2009 Accepted on: 05.07.2009 *Corresponding Author

ABSTRACT

The objective of the present investigation was to study the plasma glucose, liver glycogen, abdominal fat as well as insulin and glucagon immunoreactive (IR) cell number during annual seasonal cycle of reproduction in hibernating turtle Lissemys punctata. Plasma glucose was estimated by glucose oxidase method. Pancreatic endocrine cells were immunolocalized by Avidin-Biotin Complex method using monoclonal antibody. Glycogen localization in liver was carried out by Periodic Acid-Schiff method. L. punctata hibernate soon after reproductive period. Seasonal variations were demonstrated in plasma glucose, liver glycogen, abdominal fat and endocrine cells of pancreas. Fluctuations in plasma glucose as well as number of endocrine cells found to be dependent on physiological status of the animal. Plasma glucose found to be lowest in hibernation with concomitant rise in islet cells. The study suggested that the energy sources were available to the turtle during hibernation by mobilization of liver glycogen and abdominal fat that was associated with pancreatic endocrine cells.