The Bioscan, 4(3): 421-424, 2009
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
EVALUATION OF STREPTOKINASE AS A BLOOD CULTURE ADDITIVE FOR 
BACTERIOLOGICAL ISOLATION OF SALMONELLA ENTERICA 
SEROVER TYPHI FROM BLOOD
KAVITA NAGSHETTY, C. T. SHIVANNAVAR AND S. M. GADDAD*
*Department of P. G. Studies and Research in Microbiology,
Gulbarga University, Gulbarga - 585 106, Karnataka, INDIA
E-mail: smgaddad@gmail.com


KEY WORDS
Salmonella typhi
Clot culture
Broth culture Streptokinase
Sodium
taurocholate
Received on: 21.04.2009 Accepted on: 13.06.2009 *Corresponding Author

ABSTRACT

A total of 1200 blood samples from patients in an area endemic for enteric fever were cultured in parallel in bile salt broth with and without streptokinase. A total of 89 specimens were positive for Salmonella spp., 84 for Salmonella typhi and 5 for Salmonella paratyphi A in one or both bottles of a set. Significantly higher rates of recovery were obtained from the streptokinase-containing medium upon sub culturing blindly at 24h and 3 days of incubation. Subcultures performed at 7, 14, and 21 days also yielded a greater number of positive cultures with streptokinase than without it, although the differences between the two media were not significant. Neither of the media yielded 100% of the positive cultures. The data indicate that streptokinase aids in early recovery of S.typhi and S.paratyphi A from blood cultures, and additionally, that under the conditions used in the study, incubation beyond a 1-week period is required for efficient isolation of these organisms from blood.